Journal: Biophysical Journal

Article Title: Distinct Binding Interactions of α 5 β 1 -Integrin and Proteoglycans with Fibronectin

doi: 10.1016/j.bpj.2019.07.002

Figure Lengend Snippet: A schematic diagram of energy landscapes for protein unbinding. Two energy minima ( upper curve ) were observed for the fibronectin– α 5 β 1 -integrin interaction, but only one minimum ( lower curve ) was observed for fibronectin with the two PGs studied. ΔG represents the height of a barrier of width χ B . Here, (i) and (o) indicate the inner and outer barriers. Both curves have the same energy at large separations, so they are shifted for clarity. To see this figure in color, go online

Article Snippet: Recombinant human decorin, recombinant human α 5 β 1 -integrin, and recombinant human SDC4 were purchased from R&D Systems (Minneapolis, MN).

Techniques:

Journal: Biophysical Journal

Article Title: Distinct Binding Interactions of α 5 β 1 -Integrin and Proteoglycans with Fibronectin

doi: 10.1016/j.bpj.2019.07.002

Figure Lengend Snippet: Representative force spectroscopy data for the unbinding of SDC4 ( top ), decorin ( middle ), and α 5 β 1 -integrin ( bottom ) from a fibronectin-immobilized surface. The retraction speeds for these data are 2, 1, and 3 μ m/s, respectively. For clarity, the data for SDC4 and decorin are offset by 200 and 100 pN, respectively. To see this figure in color, go online

Article Snippet: Recombinant human decorin, recombinant human α 5 β 1 -integrin, and recombinant human SDC4 were purchased from R&D Systems (Minneapolis, MN).

Techniques: Force Spectroscopy

Journal: Biophysical Journal

Article Title: Distinct Binding Interactions of α 5 β 1 -Integrin and Proteoglycans with Fibronectin

doi: 10.1016/j.bpj.2019.07.002

Figure Lengend Snippet: Frequency distributions for extracted rupture forces, taken at different velocities for the unbinding of fibronectin from α 5 β 1 -integrin ( A ), SDC4 ( B ), and decorin ( C ). The number of measurements, n , from which the distributions were obtained, is shown in each panel. The fits (shown) are to either Gaussian or log-Gaussian models, and the modal averages of the rupture force for the corresponding loading rate were obtained. (These may be distinguished by noting that the fit to a log-Gaussian model passes through the origin.) Included errors correspond to the width of the 95% confidence interval for the fit. Standard error values for each fit, provided by statistical software package GraphPad, were significantly smaller than the error values (95% confidence interval width) quoted here. The log-Gaussian fit was used when the Gaussian fit was unsatisfactory, although the modal average is model independent. To see this figure in color, go online.

Article Snippet: Recombinant human decorin, recombinant human α 5 β 1 -integrin, and recombinant human SDC4 were purchased from R&D Systems (Minneapolis, MN).

Techniques: Software

Journal: Biophysical Journal

Article Title: Distinct Binding Interactions of α 5 β 1 -Integrin and Proteoglycans with Fibronectin

doi: 10.1016/j.bpj.2019.07.002

Figure Lengend Snippet: The dynamic force spectrum describing the unbinding between fibronectin and α 5 β 1 -integrin ( A ), SDC4 ( B ), and decorin ( C ). The linear increase of the average rupture force with the logarithm of the average loading rate for each pulling velocity, fitted using the Bell-Evans relation , is shown. Included errors correspond to the width of the 95% confidence interval from which average rupture forces were extracted. Two energy barriers were observed for the unbinding of fibronectin from α 5 β 1 -integrin.

Article Snippet: Recombinant human decorin, recombinant human α 5 β 1 -integrin, and recombinant human SDC4 were purchased from R&D Systems (Minneapolis, MN).

Techniques:

Journal: Biophysical Journal

Article Title: Distinct Binding Interactions of α 5 β 1 -Integrin and Proteoglycans with Fibronectin

doi: 10.1016/j.bpj.2019.07.002

Figure Lengend Snippet: Extracted Energetics for Each Energy Barrier Revealed in the Dynamic Spectra for the Unbinding of Fibronectin from Both the Inner, I, and Outer, o, Barriers of α 5 β 1 -Integrin, as well as Those for SDC4 and Decorin

Article Snippet: Recombinant human decorin, recombinant human α 5 β 1 -integrin, and recombinant human SDC4 were purchased from R&D Systems (Minneapolis, MN).

Techniques: